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Inhibition of Chloroplast Reactions with Phenylmercuric Acetate ^1,2

^a Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907

Phenylmercuric acetate is a selective inhibitor of the photosynthetic activities of isolated spinach (Spinacia oleracea) chloroplasts. At 5 µM concentration of phenylmercuric acetate, photophosphorylation is inhibited. At 33 µM phenylmercuric acetate, ferredoxin is inactivated. Ferredoxin-NADP oxidoreductase is 50% inhibited at 100 µM phenylmercuric acetate. Photosystem II reactions are 50% inhibited at 150 µM phenylmercuric acetate and very much higher cooncentrations—500 µM—are needed to approach complete inhibition. Phenylmercuric acetate inhibition of photosystem II appears to be selective, blocking a site between the 3-(3,4-dichlorophenyl)-1,1-dimethyl urea sensitive site and the site inactivated by high concentrations of tris buffer.

¹ Publication No. 4513 of the Purdue Agriculture Experiment Station.

² This work was supported by National Science Foundation Grant GB-27466.