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Sterol Changes during Germination of Nicotiana tabacum Seeds ¹

^a Department of Agronomy, University of Kentucky, Lexington, Kentucky 40506

The identity, composition, and concentration of the total, free, esterified, and glycosidic sterol fractions were determined during germination of tobacco seeds. The total, free, and esterified sterols increased, with stigmasterol and campesterol accounting for most of the increase. Steryl glycosides decreased during germination, and stigmasteryl and sitosteryl glycosides showed the largest decrease. During germination, sitosterol was the major sterol in all fractions but stigmasterol and campesterol showed the greatest changes. The fatty acid composition of the steryl esters and acylated steryl glycosides most closely resembled the di- and triglycerides.

¹ This work was part of Ph.D thesis of Parshall B. Bush and was supported in part by Crops Research Division, Agricultural Research Service, United States Department of Agriculture Contract 12-14-100-9559 (34). This paper (No. 71-3-30) is part of a project of the Kentucky Agricultural Experiment Station and is published with approval of the Director.

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