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Effect of Light and Gibberellin on Ribonucleic Acid Species of Pea Stem Tissues as Studied by Deoxyribonucleic Acid-Ribonucleic Acid Hybridization ¹

^a Department of Botany, University of Washington, Seattle, Washington 98195

The ability of gibberellin and light to alter gene transcription in dwarf pea (Pisum sativum L., var. Progress No. 9) stem issues has been investigated by means of DNA/RNA hybridization-competition techniques. Distinct changes in hybridizable RNA are caused by a 24-hour pretreatment of the seedlings with light, but no changes in RNA were detected up to 50 hours after treatment of the seedlings with gibberellin. Gibberellin is similar to auxin in its ability to induce stem growth without causing detectable changes in hybridizable RNA.

¹ This research was supported by Atomic Energy Commission Contract AT(45-1)-2225-T19 to Robert Cleland and by an National Defense Education Act Title IV predoctoral fellowship to William F. Thompson.