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Mitochondrial Tyrosyl Transfer Ribonucleic Acid in Soybean Seedlings

¹ Department of Botany, University of Illinois, Urbana, Illinois 61801

Soybean seedlings were examined for the presence of mitochondrial tRNA. Tyrosyl transfer tRNAs from whole cells, from a well characterized mitochondrial preparation, and from a snake venom phosphodiesterase-treated mitochondrial preparation, were compared by reverse phase chromatography. It was concluded that none of the three previously reported tRNA^Tyr species were mitochondrial. Rather, a fourth tRNA^Tyr species, eluting somewhat later, was of mitochondrial origin. Mitochondrial tRNA^Tyr was chromatographically similar to Escherichia coli tRNA^Tyr.