This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (27) Citing Articles via Google Scholar Google Scholar Articles by Horine, R. K. Articles by Ruesink, A. W. Search for Related Content PubMed PubMed Citation Articles by Horine, R. K. Articles by Ruesink, A. W. Agricola Articles by Horine, R. K. Articles by Ruesink, A. W.

Articles

Cell Wall Regeneration around Protoplasts Isolated from Convolvulus Tissue Culture ¹

^a Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401

Protoplasts of Convolvulus arvensis L. tissue culture regenerated a wall-like structure within 3 days in culture. Although unusually electron dense and atypically amorphous in the electron microscope, this structure could be digested with Myrothecium cellulase but was resistant to protease, a Rohm and Haas pectinase, and a -1, 3-exoglucanase just like the original wall. A cytochemical test for callose was negative. Wall regeneration required a readily metabolized external carbon source and was not inhibited by a high concentration of cycloheximide, puromycin, or actinomycin D. Protoplast budding was correlated with the wall regeneration, and the latter was related quantitatively to the sucrose concentration in the medium. Although a concentration of 1 µM 2,4-dichlorophenoxy acetic acid is used normally for both general culture of the tissue and for wall regeneration, concentrations of 0 and 0.1 mM, which are highly deleterious to growth, have no appreciable effect on the incidence of the wall-like structure regenerated around protoplasts. The ability of protoplasts to undergo cell wall regeneration was decreased when they were cultured in the presence of proteolytic enzymes.

¹ The National Science Foundation provided support through Grant GB 8006. This material was included in a doctoral thesis submitted by R. K. Horine to the Graduate School of Indiana University.