Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 50:458-462 (1972)
© 1972 American Society of Plant Biologists

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Articles

Photoregulation of Nicotinamide Adenine Dinucleotide Kinase Activity in Cell-free Extracts 1

Takafumi Tezuka and Yukio Yamamoto2

a Biological Institute, Faculty of Science, Nagoya University, Nagoya 464, Japan

This article gives evidence that NAD kinase activity is controlled by the action of phytochrome. The NADP level rapidly increased in the cotyledons of seedlings of Pharbitis nil strain Violet (a short day plant), when the inductive dark for flowering was interrupted with a 5-minute illumination of red light. Illumination with far red light immediately after illumination with red light counteracted partly the effect of the latter.

A partially purified phytochrome preparation from the uppermost internodes including the hook of Pisum sativum var. Alaska contained NAD kinase activity, and this increased greatly on illumination with red light. The effects of red and far red light on NAD kinase activity were reversible. The Km value of NAD kinase in the phytochrome preparation for NAD was 1.84 mM in the dark and 0.90 mM under red light. A broad optimum of the NAD kinase reaction and its photopotentiation appeared in the region of pH 6.6 to 8.6.

The NAD kinase activity in a phytochrome preparation from the coleoptiles of Avena sativa was also activated by red light.

It was suggested that the Pfr type of phytochrome may contribute to the decrease of the Km value of NAD kinase reaction by causing a conformational alteration of NAD kinase.

2 To whom requests for reprints should be sent. Present address: Department of Agricultural Chemistry, Faculty of Agriculture, Nagoya University, Nagoya 464, Japan.

1 Preliminary accounts of this work have been published elsewhere (10, 16).






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