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Plant Physiology 50:473-476 (1972)
© 1972 American Society of Plant Biologists

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Articles

The Role of a D-Mannosyl-Lipid as an Intermediate in the Synthesis of Polysaccharide in Phaseolus aureus Seedlings 1

David L. Storm and W. Z. Hassid

a Department of Biochemistry, University of California, Berkeley, California 94720

Particulate preparations from Phaseolus aureus produce a D-mannosyl-lipid when treated with GDP-D-mannose. This lipid complex appears to be an active D-mannose donor, and some investigators have proposed that its role might be an obligatory intermediate in mannan synthesis of higher plants. When the partially purified D-mannosyl-lipids, isotopically labeled in the D-mannose moiety, were treated with particulate enzymes under a variety of conditions, a negligible amount of material was produced that behaved as a polysaccharide. Endogenous, particle-bound D-mannosyl-14C-lipid prepared from P. aureus particles readily transferred D-mannose to GDP to yield GDP-D-mannose and was hydrolyzed to free D-mannose when treated briefly with 0.01 N HCl at 100 C. The D-mannosyl-lipid, therefore, exhibits active D-mannose transfer potential in its endogenous state. When endogenous glycosyl-lipid was incubated in the absence of GDP-D-mannose-14C, little or no polysaccharide was produced. It was, instead, slowly degraded to D-mannose. Addition of several different unlabeled sugar nucleotides had no effect on the results. Our studies to date, therefore, offer no evidence that the mannosyl-lipid is an obligatory precursor of polysaccharide.

1 This investigation was supported in part by Research Grant A-1418 from the National Institutes of Health, United States Public Health Service, and by Research Grant GB11819 from the National Science Foundation. Support of this work by the Agricultural Experiment Station is also acknowledged.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1972 by the American Society of Plant Biologists