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Articles

Isolation and Characterization of Membranes from the Cultivated Mushroom ¹

Lee C. Schisler^b

^a Division of Food Science and Industry, The Pennsylvania State University, University Park, Pennsylvania 16802, Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania 16802

The membranes of sporophore cap tissue from the cultivated mushroom, Agaricus bisporus (Lange) Sing., were isolated using discontinuous sucrose gradient ultracentrifugation of a tissue homogenate. A membrane-rich fraction was concentrated at the 1.16/1.18 g/cc interface and a mitochondria-rich fraction at the 1.18/1.20 g/cc interface. The membrane fraction was judged to be greater than 90% membrane vesicles by electron microscopy. The protein to lipid ratio of the membrane fraction was 1.1; the molar ratio of sterol to phospholipid was 0.77. The specific radioactivity of a Mg-activated ATPase was 2.5 times greater in the membrane fraction than in the homogenate. No 5'-nucleotidase or Na-K-Mg-activated ATPase activity was observed.

¹ Authorized for publication as Paper No. 4144 on February 29, 1972, in the Journal Series of The Pennsylvania Agricultural Experiment Station.