Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 50:551-555 (1972)
© 1972 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (43)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stewart, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stewart, C. R.
Agricola
Right arrow Articles by Stewart, C. R.
Articles

Effects of Proline and Carbohydrates on the Metabolism of Exogenous Proline by Excised Bean Leaves in the Dark

Cecil R. Stewart

1 Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50010

Proline was metabolized when vacuum infiltrated into starved bean (Phaseolus vulgaris L.) leaves from plants previously in the dark for 48 hours, but an equivalent increase in protein proline was not observed. When 14C-proline was infiltrated into starved leaves, a large percentage of the 14C was recovered in other amino acids, organic acids, and CO2, in addition to that recovered as protein proline. However, extensive oxidation of proline was observed only if enough proline was added to increase substantially the endogenous concentration of proline. Increasing the endogenous concentration did not affect the amount of proline that was incorporated into protein.

When added to leaves from plants previously in the light of near saturating intensity for more than 16 hr, very little 14C-proline was oxidized, even when a sufficient amount of proline was added to increase the endogenous concentration. Adding sucrose to starved leaves along with the proline slowed down the oxidation of proline. Thus, it appears that some carbohydrate or intermediate of carbohydrate metabolism may inhibit the oxidation of proline in leaves.

Based on kinetics of labeling, the pathway of proline oxidation was by conversion to glutamic acid and subsequent metabolism to intermediates in the Krebs cycle and to CO2.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1972 by the American Society of Plant Biologists