Plant Physiol.
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Plant Physiology 51:26-30 (1973)
© 1973 American Society of Plant Biologists

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Articles

Ultrastructural Transformations in Bean Inner Mitochondrial Membranes 1

R. H. Wilson, E. L. Thurston2 and R. Mitchell

a Department of Botany, University of Texas, Austin, Texas 78712

The ultrastructure of inner membrane-matrix mitochondria isolated from bean (Phaseolus vulgaris) shoots was examined in different metabolic states. Gross ultrastructural transformations analogous to the condensed-to-orthodox configurational changes reported in mammalian mitochondria are observed on transistion from nonrespiring to respiring metabolism. With the induction of oxidative phosphorylation, the particles remain in the orthodox configurational state. The reverse orthodox-to-condensed configurational changes observed in mammalian preparations does not occur. Optically monitored absorbancy studies with bean particles show a substrate-supported Pi-induced swelling under the same conditions that induce the condensed-to-orthodox ultrastructural transformation. The swelling is associated with the net uptake of K+ and Pi as well as a small Pi-induced respiratory stimulation. When phosphorylation is initiated with these swollen particles, the optically monitored volume remains unchanged. Thus a positive correlation exists between the ultrastructural configuration and the osmotic volume changes, which supports the conclusion that configurational changes reflect internal osmotic adjustments.

2 Present address: Department of Biology, Texas A&M University, College Station, Texas 77843.

1 This investigation was supported by Biomedical Science Support Grant FR-07091-04 from the General Research Branch, Division of Research Resources, Bureau of Health Professions, Education and Manpower Training, National Institutes of Health.






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Copyright © 1973 by the American Society of Plant Biologists