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Appearance of Three Chloroplast Isoenzymes in Dark-grown Pea Plants and Pea Seeds ^1,2

^a Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680

Activity peaks characteristic of the chloroplastic Calvin cycle enzymes triose-phosphate isomerase, ribose 5-phosphate isomerase, and fructose 1,6-diphosphate aldolase are found in isoelectric focusing patterns of dark-grown pea (Pisum sativum) seedlings and seeds. Apparently, in this higher plant these three chloroplastic isoenzymes can be formed in the absence of light and of chloroplast formation.

² A preliminary report of these findings was presented at the American Society of Plant Physiology Meetings, Minneapolis, Minn., 1972 (10).