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Plant Physiology 51:409-411 (1973)
© 1973 American Society of Plant Biologists

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Possible Errors in Quantitative Determination of Phenylalanine Ammonia-Lyase Activity by Spectrophotometric Methods 1

A. Erez

a Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.

Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of {alpha}-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.

1 Contribution from the Volcani Center, Agricultural Research Organization, Bet Dagan, Israel. 1972 Series, No. 2181-E.






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Copyright © 1973 by the American Society of Plant Biologists