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Plant Physiology 51:591-593 (1973)
© 1973 American Society of Plant Biologists

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Articles

Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa1

Robert H. Schloemer and Reginald H. Garrett

a Department of Biology, University of Virginia, Charlottesville, Virginia 22903

The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO3– in the NADH-nitrate reductase assay are 10 µM, 2.6 µM, and 0.23 mM, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.

1 This work was supported in varying degrees by Grants GMO-1450, GM-13201, and AM-18955 from the National Institutes of Health.






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