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Mechanism of Naphthaleneacetic Acid Conjugation

No Effect of Ethylene ¹

^a Department of Horticulture, Michigan State University, East Lansing, Michigan 48823

Formation of naphthaleneacetic acid-glucose (NAGLu) in detached leaves, floating on -naphthaleneacetic acid-1-¹⁴C (NAA, 0.05 microcurie per milliliter, 3.1 µM)-buffer solution (phosphate-citrate, pH 4.2) began immediately while there was a 2- to 4-hour lag before NAA-asparatate (NAAsp) could be detected. Subsequent increase in the NAAsp conjugate reflected a decrease in free NAA to 1 to 2% of the total radioactivity taken up. Pretreatment with 31 µM¹²C-NAA for 18 hours doubled NAAsp formation after transfer for 4 hours to ¹⁴C-NAA. Pretreatment with ethylene, as ethephon (up to 400 milligrams per liter) or ethylene gas (10 microliters per liter), did not induce NAAsp formation. In the presence of NAA, ethylene had no effect on NAA conjugation. Similarly, CO₂ (5%) did not modify the formation of the conjugates. Rhizobitoxine (1.87 µM) inhibited NAA-induced ethylene production but did not prevent NAA-induced formation of NAAsp. We concluded that the conjugation of NAA with aspartic acid is not mediated by ethylene.

¹ This study was supported by United States Public Health Service Grant CC 00246 from the National Communicable Disease Center, Atlanta, Ga., and Food and Drug Administration Grant FD 00223. Michigan Agricultural Experiment Station Journal Article 6094.

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