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Plant Physiology 51:1051-1054 (1973)
© 1973 American Society of Plant Biologists

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Articles

Enzymatic Fractionation of Carbon Isotopes by Phosphoenolpyruvate Carboxylase from C4 Plants 1

Tom Whelana and W. M. Sacketta

C. R. Benedictb

a Department of Oceanography, Texas A & M University, College Station, Texas 77843, Department of Plant Sciences, Texas A & M University, College Station, Texas 77843

The carbon atoms of glucose and malate in C4 plants are 2 to 3{per thousand} enriched in 12C with respect to atmospheric CO2; whereas these intermediates in C3 plants are 15 to 18{per thousand} enriched with 12C with respect to atmospheric CO2. The enzymatic synthesis of malate from phosphoenolpyruvate and bicarbonate in preparations of leaves of Sorghum bicolor, Haygrazer result in a carbon isotope fractionation of about 3{per thousand}. The enzymatic synthesis of phosphoglyceric acid from ribulose 1,5-diP and CO2 in these preparations (contaminated with carbonic anhydrase) at 24 C and 37 C result in a carbon isotope fractionation of 33.7{per thousand} and 18.3{per thousand}, respectively. These data are consistent with the conclusion that the small enrichment of 12C in the carbon atoms of malate and glucose (with respect to atmospheric CO2) in leaves of Sorghum bicolor, Haygrazer occurs at the phosphoenolpyruvate carboxylase step.

1 Acknowledgement is made to the donors of the Petroleum Research Fund, administered to the American Chemical Society, for partial support of this research and to the Robert A. Welch Foundation for partial support of this research by grants to W.M.S. and C.R.B.




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