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Plant Physiology 51:1109-1116 (1973)
© 1973 American Society of Plant Biologists

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Articles

Separation and Ultrastructure of Proplastids from Dark-grown Euglena Cells

Ilana Ophir and Yehuda Ben-Shaul

Laboratory for Electron Microscopy, Tel-Aviv University, Tel-Aviv, Israel

A procedure for the separation of proplastids free of mitochondria from dark-grown Euglena cells has been developed. A fraction enriched in proplastids was used for freeze-etching study of proplastid structure. The prolamellar body in freeze-etched replicas appeared sponge-like, with thylakoids, often vesicular, emerging from it. The prolamellar body and the thylakoids were covered by particles of about 100Å in diameter. No larger particles, typical of light-grown chloroplasts, were observed.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1973 by the American Society of Plant Biologists