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Hormonal Control of Orthophosphate Incorporation into Phospholipids of Barley Aleurone Layers ¹

^a Michigan State University-Atomic Energy Commission Plant Research Laboratory and Department of Biochemistry, Michigan State University, East Lansing, Michigan 48823

Gibberellic acid added to isolated barley aleurone layers enhances orthophosphate incorporation into chloroform-methanol-soluble compounds. The effect is measurable at 4 to 6 hours after the addition of gibberellic acid and reaches a maximum after 8 to 12 hours. The increase in the rate of orthophosphate incorporation is 3- to 5-fold over the rate in control layers incubated without gibberellic acid.

The gibberellic acid enhancement of the rate of phospholipid labeling is inhibited within 1 to 2 hours by cycloheximide, 6-methylpurine, and abscisic acid.

The increase in labeling of phospholipids occurs throughout the subcellular fractions rather than being restricted to a specific fraction or organelle. The increase in radioactivity in phospholipids as shown by thin layer chromatography is due to a proportional increase in all phospholipids.

The enhancement by gibberellic acid of the rate of phospholipid labeling may be required for the subsequent production of gibberellic acid-induced hydrolases.

¹ This research was supported by the United States Atomic Energy Commission under Contract AT(11-1)-1338 and by Grant GB-8774 from the National Science Foundation to J. E. V. This manuscript is based on a dissertation submitted to Michigan State University by D. E. K. in partial fulfillment of the requirements for the Ph.D. degree. This is Journal Article 6022 from the Michigan Agricultural Experiment Station.

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