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Plant Physiology 52:233-235 (1973)
© 1973 American Society of Plant Biologists

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Articles

Cholinesterases from Plant Tissues

II. Inhibition of Bean Cholinesterase by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride (AMO-1618) 1

J. Riov2 and M. J. Jaffe

a Department of Botany, Ohio University, Athens, Ohio 45701

2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride (AMO-1618) inhibits the activity of a cholinesterase isolated from mung bean (Phaseolus aureus) roots at concentrations comparable to those which retard growth and inhibit development of secondary roots of bean seedlings. Fifty per cent inhibition of the cholinesterase activity occurred at 0.21 mM. Inhibition of the cholinesterase is non-competitive and irreversible. The growth retardant seems to be a specific cholinesterase inhibitor since it has no effect on non-choline esterases.

2 Permanent address: Department of Citriculture, Hebrew University, Rehovoth, Israel.

1 This work was supported by National Science Foundation Grant GB 20474 to M. J. J.






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Copyright © 1973 by the American Society of Plant Biologists