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Phosphoenolpyruvate Carboxykinase in Plants Exhibiting Crassulacean Acid Metabolism ¹

^a Department of Biochemistry, University of Georgia, Athens, Georgia 30602

Phosphoenolpyruvate carboxykinase has been found in significant activities in a number of plants exhibiting Crassulacean acid metabolism. Thirty-five species were surveyed for phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxylase, ribulose diphosphate carboxylase, malic enzyme, and malate dehydrogenase (NAD). Plants which showed high activities of malic enzyme contained no detectable phosphoenolpyruvate carboxykinase, while plants with high activities of the latter enzyme contained little malic enzyme. It is proposed that phosphoenolpyruvate carboxykinase acts as a decarboxylase during the light period, furnishing CO₂ for the pentose cycle and phosphoenolpyruvate for gluconeogenesis.

Some properties of phosphoenolpyruvate carboxykinase in crude extracts of pineapple leaves were investigated. The enzyme required Mn²⁺, Mg²⁺, and ATP for maximum activity. About 60% of the activity could be pelleted, along with chloroplasts and mitochondria, in extracts from leaves kept in the dark overnight.

³ To whom inquiries should be addressed.

¹ This research was supported in part by National Science Foundation Grant GB 20661 and by a fellowship grant to P. Dittrich from Deutsche Forschungsgemeinschaft.

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