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Activation and Inhibition of Ribulose 1,5-Diphosphate Carboxylase by 6-Phosphogluconate ¹

^a Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720

Ribulose 1,5-diphosphate carboxylase, when activated by preincubation with 1 mM bicarbonate and 10 mM MgCl₂ in the absence of ribulose 1,5-diphosphate, remains activated for 20 minutes or longer after reaction is initiated by addition of ribulose diphosphate. If as little as 50 µM 6-phosphogluconate is added during this preincubation period, 5 minutes before the start of the reaction, a further 188% activation is observed. However, addition of 6-phosphogluconate at the same time or later than addition of ribulose diphosphate, or at any time with 50 mM bicarbonate, gives inhibition of the enzyme activity. Possible relevance of these effects in vivo regulatory effects is discussed.