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Plant Physiology 52:604-607 (1973)
© 1973 American Society of Plant Biologists

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Polyribosome Formation and Protein Synthesis in Imbibed but Dormant Lettuce Seeds 1

David W. Fountain and J. Derek Bewley

a Department of Biology, The University of Calgary, Calgary, Alberta, Canada T2N 1N4

Dormancy is maintained in Grand Rapids lettuce (Lactuca sativa) seeds imbibed on water in darkness at 25 C. Polyribosome formation and protein synthesis occur early in the imbibition phase and considerable polysomal material is also present after 24 and 48 hours, even though the seeds have failed to germinate. Incorporation of labeled leucine into protein following a 24-hour preincubation period shows that these polysomes are active in protein synthesis.

1 This work was supported by a National Research Council of Canada Grant A-6352 to J.D.B. and an equipment grant from the University of Calgary. D.W.F. gratefully acknowledges support in the form of a National Research Council of Canada postgraduate scholarship.






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Copyright © 1973 by the American Society of Plant Biologists