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Plant Physiology 52:627-632 (1973) © 1973 American Society of Plant Biologists The Formation, Isolation, and Biological Activity of a Cytokinin 7-Glucoside 1,2a Departments of Biochemistry, Botany, and Medicinal Chemistry, The University of Kansas, Lawrence, Kansas 66044
The cytokinin, 6-benzylaminopurine, is converted to its 7-glucoside in intact seedlings, organ slices, and tissue cultures from several plants. The ribonucleoside and 5'-ribonucleotide appear transiently, and the general metabolic sequence seems to be nearly identical in the four plant species thus far studied. The glucoside persists for long periods in plant tissues, while all other forms of the cytokinin are rapidly metabolized and disappear within a few hours. A procedure for the isolation in pure form of the glucoside is described.
3 Present address: Lackland Air Force Base, Orlando, Fla. 4 Present address: Departamento de Biologia, Universidad De Oriente, Cumana, Venezuela. 5 Present address: Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada. 6 Present address: Department of Botany, A. Mickiewicz University, Poznan, Poland. 7 Present address: Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kan. 8 Present address: Departamento de Ciencias Basicas, Universidad de San Marcos, Lima, Peru. 1 This work was supported in part by National Science Foundation Grant GB8319, National Institutes of Health Grant HD09835, and Biomedical Sciences Support Grant 4837-5706-6. 2 This material is in part from theses presented by James Cornette, Guy Deleuze, William Dyson, Paul Niu, and Javier Zapata in partial fulfillment of the requirements for the M.S. or Ph.D. degree at the University of Kansas.
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