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Articles

High Energy Charge as a Requirement for Axis Elongation in Response to Gibberellic Acid and Kinetin during Stratification of Acer saccharum Seeds ¹

^a Department of Biology, University of Waterloo, Waterloo, Ontario, Canada

The growth potential of embryonic axes of Acer saccharum Marsh. increased during moist storage at 5 C but not at 20 C. During the period of increasing growth potential, the oxygen consumption of the axes remained constant. It was possible to distinguish three phases of the stratification-germination process at 5 C with respect to response of the axis to gibberellic acid and kinetin. From 0 to 10 days the growth regulators had no effect on elongation; from 10 to 60 days axis elongation was stimulated; and between day 60 and day 75, when germination had begun, the growth substances were inhibitory. The adenylate energy charge remained low (0.15) in axes of dry dormant seeds but increased to 0.78 following imbibition of water and 10 days of moist storage at 5 C. This phenomenon was not specifically related to low temperature stratification, since a rapid increase in the energy charge of the axes also occurred following imbibition and moist storage at 20 C. The excised axes would elongate in response to the growth substances only when a high energy charge (approximately 0.8) was maintained.

¹ This research was supported in part by a grant from the National Research Council of Canada to E. B. Dumbroff. A preliminary report of these findings was presented at the American Society of Plant Physiology Meetings, Minneapolis, 1972.