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Articles

Wound Healing in Potato Tuber Tissue

Phosphon Inhibition of Developmental Processes Requiring Protein Synthesis ¹

^a Department of Botany, University of Kansas, Lawrence, Kansas 66044, Department of Physiology and Cell Biology, University of Kansas, Lawrence, Kansas 66044, ^c Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66044

Several aspects of wound healing in tuber tissue of potato (Solanum tuberosum var. Kennebec), known to require protein synthesis, are inhibited by 2,4-dichlorobenzyltributylphosphonium chloride (Phosphon D). Cell division was completely blocked by 60 µM Phosphon and markedly reduced by concentrations as low as 3 µM. When applied at the time of wounding, 0.25mM Phosphon completely prevented the wound-induced respiratory increase. Application at 15 hours after wounding arrested respiration at the rate present at that time. The same concentrations of Phosphon inhibited auxin-induced cell expansion of the tissue, protein synthesis as measured by the incorporation of leucine-¹⁴C into the trichloroacetic acid-insoluble fraction of tissue disks, and the appearance of wound-induced peroxidase isozymes. None of these inhibitory effects of Phosphon could be prevented or reversed by the application of gibberellic acid. All wound-induced processes inhibited by Phosphon are also inhibited by cycloheximide. It is suggested that inhibitory effects of Phosphon on wound healing in potato and on other developmental processes in excised plant tissues which cannot be reversed by gibberellin are due to interference with protein synthesis.