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Plant Physiology 53:250-257 (1974)
© 1974 American Society of Plant Biologists

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Isolation and Partial Characterization of Four Host-specific Toxins of Helminthosporium maydis (Race T) 1

Arthur L. Karr, Jr.2, Dale B. Karr and Gary A. Strobel3

a Department of Plant Pathology, Montana State University, Bozeman, Montana 59715

Helminthosporium maydis, race T, produces four host-specific toxins in culture. These have been designated toxins I, II, III, and IV. A method for isolation and purification of the four toxins is presented, and the criteria of purity of preparations of toxins I, II, and III are given. Toxins I and II are chemically similar and yield the same molecular ion when subjected to mass spectrometry, while toxin III appears to be a glycoside of a compound related to toxins I and II. Toxins I, II, and III can be biologically derived from 14C-mevalonic acid or 14C-acetate, permitting preparation of 14C-labeled toxins. Some chemical, spectral, and chromatographic properties of toxins I, II, and III are presented, and these data are discussed relative to the possible structure of the three compounds. In addition, four host-specific toxins have been isolated from corn infected with H. maydis (race T). These toxins are recovered in the same fractions as toxins I, II, III, and IV using the isolation procedure described here. Three of the toxins isolated from infected corn cannot be distinguished from toxins I, II, and III on the basis of infrared spectra or chromatographic mobility.

2 A National Institutes of Health Post Doctoral Fellow.

3 Public Health Service Research Career Development Awardee 1K4-GM42, 475-05 from the National Institute of General Medical Sciences.

1 This work was supported in part by National Science Foundation Grant GB 12956, United States Department of Agriculture Cooperative States Research Service Grant C145-2575, and is paper No. 453 of the Montana Agricultural Experiment Station.






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Copyright © 1974 by the American Society of Plant Biologists