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Cytochemical Localization of Catalase Activity in Glyoxysomes from Castor Bean Endosperm ¹

Jürg Graf^b

^a Institut für Allgemeine Biochemie, Universität Wien, and Ludwig-Boltzmann-Forschungsstelle für Biochemie, A-1090 Wien, Austria, Institut für Allgemeine und Experimentelle Pathologie, Universität Wien, A-1090 Wien, Austria

Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H₂O₂ is distributed uniformly throughout the matrix of the organelles. Ricinosomes or dilated cisternae may be completely absent from the organelle preparation or are, at the most, a minor contaminant.