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Plant Physiology 53:317-318 (1974)
© 1974 American Society of Plant Biologists

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Soluble Peroxidase in Fluid from the Intercellular Spaces of Tobacco Leaves 1

W. G. Rathmell and Luis Sequeira

a Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706

A high proportion of the soluble peroxidase of tobacco (Nicotiana tabacum L. var. Bottom Special) leaves is found in the fluid obtained by centrifugation of a buffer solution previously infiltrated into the intercellular spaces. Only a very small amount of the cytoplasmic enzyme, glucose 6-phosphate dehydrogenase, is present in this fluid. Specific activity data suggest that an active process is responsible for the transfer of soluble peroxidase to the intercellular space and that the intercellular fluid fraction is not simply composed of material moving out of leaf cells by diffusion. The centrifugation method is a satisfactory means of isolating diluted intercellular fluid for biochemical and physiological investigations.

1 This work was supported by a grant from the National Science Foundation. W.G.R. received a travel grant from the Agricultural Research Council Wain Fund.




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Copyright © 1974 by the American Society of Plant Biologists