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Characterization of a H⁺ Efflux from Suspension-cultured Plant Cells ¹

^a Department of Chemistry, University of Colorado, Boulder, Colorado 80302

A readily assayed H⁺ efflux from sycamore (Acer pseudoplatanus), rye (Lolium perenne), and bean (Phaseolus vulgaris cultivars Red Kidney and Small White) suspension-cultured cells has been detected and partially characterized. The H⁺ efflux has been shown to require a source of energy, to be significantly stimulated by Na⁺ and Mg²⁺ but not by K⁺ and Ca²⁺, and to have a pH optimum at 7. The study of this H⁺ efflux was undertaken because the characteristics of auxin-induced growth and of H⁺-induced growth are sufficiently similar to suggest that a H⁺ efflux may be an intermediate in the mechanism of auxin-induced growth. However, the H⁺ efflux from these suspension-cultured cells was found to be insensitive to exogenously added hormones.