This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (100) Citing Articles via Google Scholar Google Scholar Articles by Mackender, R. O. Articles by Leech, R. M. Search for Related Content PubMed PubMed Citation Articles by Mackender, R. O. Articles by Leech, R. M. Agricola Articles by Mackender, R. O. Articles by Leech, R. M.

Articles

The Galactolipid, Phospholipid, and Fatty Acid Composition of the Chloroplast Envelope Membranes of Vicia faba. L. ¹

^a Department of Biology, University of York, Heslington, York, England

The galactolipid, phospholipid, and fatty acid composition of chloroplast envelope membrane fractions isolated from leaves of Vicia faba L. has been determined. The major lipids in this fraction are: monogalactosyldiglyceride, 29%; digalactosyldiglyceride, 32%; phosphatidylcholine, 30%; and phosphatidylglycerol 9%. The lipid composition of the chloroplast envelope membranes is qualitatively similar to that of the lamellar membranes isolated from the same plastids, but the proportion of each lipid present is very different. The total galactolipid to total phospholipid ratio was 1.6: 1 in the envelope and 11.1: 1 in the lamellae. The monogalactosyldiglyceride-digalactosyl-diglyceride ratio was 0.9: 1 in the envelope and 2.4: 1 in the lamellae. Both membranes lack phosphatidylethanolamine.

Linolenic acid is the major fatty acid in the envelope lipids representing 63% of the total fatty acid, whereas in the lamellae it represents 83%. The same fatty acids are present in both the envelope and lamellar lipids except the trans-³-hexadecenoic acid, which is confined to the lamellar lipids, particularly the phospholipid fraction.

A quantitative comparison of the lipid and fatty acid compositions of the envelope with those of mitochondrial and microsomal fractions indicates that the chloroplast envelope has a composition intermediate between that of the chloroplast lamellae and these extrachloroplastic membranes.

¹ This work was supported by the Science Research Council, Grant B/SR/8692 and by an Imperial Chemical Industries fellowship to R.O.M.

This article has been cited by other articles:

				H.-H. Kunz, M. Scharnewski, K. Feussner, I. Feussner, U.-I. Flugge, M. Fulda, and M. Gierth The ABC Transporter PXA1 and Peroxisomal {beta}-Oxidation Are Vital for Metabolism in Mature Leaves of Arabidopsis during Extended Darkness PLANT CELL, September 1, 2009; 21(9): 2733 - 2749. [Abstract] [Full Text] [PDF]

				E. Schleiff, R. Tien, M. Salomon, and J. Soll Lipid Composition of Outer Leaflet of Chloroplast Outer Envelope Determines Topology of OEP7 Mol. Biol. Cell, December 1, 2001; 12(12): 4090 - 4102. [Abstract] [Full Text] [PDF]