Plant Physiol. Drug Metab Dispos
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Plant Physiology 53:688-690 (1974)
© 1974 American Society of Plant Biologists

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Use of Protein in Extraction and Stabilization of Nitrate Reductase 1

L. E. Schrader, D. A. Cataldo and D. M. Peterson

a Department of Agronomy and United States Department of Agriculture, Agricultural Research Service, University of Wisconsin-Madison, Madison, Wisconsin 53706

The in vitro instability of nitrate reductase (EC 1.6.6.1) activity from leaves of several species of higher plants was investigated. Decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. The rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. Activity was relatively stable in certain genotypes of Zea mays L., but extremely unstable in others. In all genotypes of Avena sativa L. and Nicotiana tabacum L. studied, nitrate reductase was unstable. Addition of 3% (w/v) bovine serum albumin or casein to extraction media prevented or retarded the decay of nitrate reductase activity for several hours. In addition, the presence of bovine serum albumin or casein in the enzyme homogenate markedly increased nitrate reductase activity (up to 15-fold), especially in older leaf tissue.

1 This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, and by United States Department of Agriculture Cooperative Agreement 12-14-100-10,888.






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