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Plant Physiology 53:694-698 (1974) © 1974 American Society of Plant Biologists Isoaccepting Transfer Ribonucleic Acids during Chilling Stress in Soybean Seedling Hypocotyls 1,2a School of Forestry, University of Missouri, Columbia, Missouri 65201
Total aminoacylation of glycine and leucine transfer RNAs was compared between chilled and nonchilled hypocotyls of 7-day-old soybean seedlings. Total charging was greater for both specific transfer RNAs from nonchilled sources. Isoaccepting transfer RNA species for glycine and leucine were fractionated using reverse phase column chromatography. Leucyltransfer RNAs were fractionated into six distinct fractions with relatively small shifts appearing in specific fractions between chilled and nonchilled sources. Glycyl-transfer RNAs were fractionated into two distinct fractions with major shifts appearing for both fractions between chilled and nonchilled sources.
3 Present address: No. 15, Lane 4, Shyh-Yuan Rd., Gou Tzyy Koo, Taipei, 116, Taiwan. 1 This investigation was supported in part by National Science Foundation Grant GB 8692. 2 Contribution from the Missouri Agricultural Experiment Station; Journal Series No. 6775.
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