Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 53:752-758 (1974)
© 1974 American Society of Plant Biologists

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Articles

Cholinesterases from Plant Tissues

III. Distribution and Subcellular Localization in Phaseolus aureus Roxb. 1

R. A. Fluck and M. J. Jaffe

a Department of Botany, Ohio University, Athens, Ohio 45701

The distribution and localization of cholinesterase in Phaseolus aureus, Glycine max, and Pisum sativum is described. The enzyme is present in roots, leaves, stems, root callus tissue, root cells suspension cultures, and root nodules. Cholinesterase in roots is found primarily in the cell wall. In cell fractionation experiments, at least 95% of the cholinesterase activity is associated with cell wall material. The enzyme can be solubilized by salt solutions, whereas Triton X-100 and sodium deoxycholate solubilize relatively small amounts of the enzyme. Cytochemical techniques have been employed to show the presence of cholinesterase activity at the cell surface and in the cell wall of certain cells of the root.

1 This work was supported by National Science Foundation Grants GB20474 and GB33257 to M. J. J., and a National Science Foundation Postdoctoral Fellowship and an Ohio University Research Council Grant to R. A. P.




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Y. Sagane, T. Nakagawa, K. Yamamoto, S. Michikawa, S. Oguri, and Y. S. Momonoki
Molecular Characterization of Maize Acetylcholinesterase. A Novel Enzyme Family in the Plant Kingdom
Plant Physiology, July 1, 2005; 138(3): 1359 - 1371.
[Abstract] [Full Text] [PDF]


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