Plant Physiol.
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Plant Physiology 53:759-763 (1974)
© 1974 American Society of Plant Biologists

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Articles

An Apparent Cellulase Complex in Tomato (Lycopersicon esculentum L.) Fruit 1

Francis E. Sobotka2 and David A. Stelzig

a Division of Plant Sciences, West Virginia University, Morgantown, West Virginia 26506

Four enzyme-containing fractions were separated by ammonium sulfate fractionation of 2-day, postbreaker tomato (Lycopersicon esculentum L. cv. Manhattan). The pH optima and substrate specificities are reported. The enzymes were identified as a nonspecific {beta}-glucosidase, an exo-{beta}-1,4-glucanase, and two endocellulases. Both endocellulase fractions were able to catalyze the hydrolysis of various insoluble cellulose materials.

Together, the enzymes studies are capable of completely degrading insoluble cellulose to short chain cellodextrins and glucose. Ripening tomato fruit apears to contain a complex of cellulases similar to that of cellulolytic bacteria and fungi.

2 Present address: Research and Development, Hunt Wesson Foods, Inc., P. O. Box 107, Perrysburg, Ohio 43551.

1 Manuscript published with the approval of the Director of the West Virginia Agricultural Experiment Station, Morgantown, as Scientific Paper No. 1281.






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Copyright © 1974 by the American Society of Plant Biologists