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Plant Physiology 53:866-869 (1974)
© 1974 American Society of Plant Biologists

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Isolation and Characterization of Microbodies from the Alga Chlorogonium elongatum1

Helmut Stabenau2 and Harry Beevers

a Division of Natural Sciences, University of California, Santa Cruz, California 95064

The alga Chlorogonium elongatum was grown autotrophically or heterotrophically on acetate. Cells harvested in the logarithmic phase of growth were disrupted, and the whole homogenates were fractionated on sucrose gradients. Protein and enzyme determinations carried out on the fractions led to the following conclusions. Chloroplast fragments which represent the major portion of particulate protein in autotrophic cells migrate to density 1.17 g/cm3. In heterotrophic cells, mitochondria comprise most of the particulate protein, and these particles accumulate at density 1.19 g/cm3, as shown by a peak of cytochrome oxidase in this region. Part of the catalase and uricase, two marker enzymes for microbodies, were found in the soluble fractions, but 60% or more of these activities were recovered at density 1.225 g/cm3 from autotrophic cells. Electron micrographs showed that in this region there were microbodies with a diameter of 0.4 micrometer. The isolated microbodies contained no isocitrate lyase, a marker enzyme of glyoxysomes. This enzyme was completely soluble and therefore seems not to be associated with organelles in this organism.

2 Present address: Pflanzenphysiologisches Institut, 34 Göttingen, Untere Karspüle 2, Germany.

1 This work was supported by National Science Foundation Grant GB 35376X1 and the Deutsche Forschungsgemeinschaft.






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Copyright © 1974 by the American Society of Plant Biologists