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Plant Physiology 54:57-59 (1974)
© 1974 American Society of Plant Biologists

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Articles

The Respiratory Chain of Chlorella protothecoides

II. Isolation and Characterization of Mitochondria 1

Neil G. Grant2 and Max H. Hommersand

a Department of Botany, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514

Mitochondria were isolated from glucose-bleached Chlorella protothecoides Krüger, Indiana strain 25. These mitochondria oxidized succinate, NADH, and L-malate at high rates. Oxygen uptake with these substrates was partially inhibited by 1 mMm-chlorobenzhydroxamic acid (mCLAM). Respiratory control was seen with succinate as substrate in the presence of mCLAM. The apparent Km for succinate was determined to be 0.83 mM. Chlorella mitochondria catalyzed the oxidation of reduced horse heart cytochrome c.

2 National Aeronautics and Space Administration predoctoral fellow. Present address: Department of Biology, The City College, New York, N. Y. 10031.

1 This work was supported by an American Cancer Society Institutional Subgrant. This paper is a portion of a dissertation submitted to the University of North Carolina by N. G. Grant.






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Copyright © 1974 by the American Society of Plant Biologists