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Plant Physiology 54:60-66 (1974)
© 1974 American Society of Plant Biologists

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Change in Invertase Activity of Sweet Potato in Response to Wounding and Purification and Properties of Its Invertases 1

Kazunobu Matsushita and Ikuzo Uritani

a Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan

When root tissue of sweet potato (Ipomoea batatas Lam.) was sliced, acid invertase activity, initially absent in freshly sliced tissue, appeared after a 3- to 6-hour lag phase, rapidly reached a maximum in 18 hours, and thereafter decreased. The increase in invertase activity was accompanied by a decrease in sucrose content of the root tissue. Alkaline invertase activity was present in fresh root tissue, but changed little after wounding. Acid invertase in wounded tissue and alkaline invertase in fresh tissue were purified and their properties were investigated. The acid invertase was a ß-fructofuranosidase and was unaffected by substrate or by any of the cations and several metabolites. The alkaline invertase was more specific for sucrose, was inhibited by glucose and glucose 6-phosphate, and displayed non-Michaelis-Menten kinetics.


1 This paper constitutes Part 111 of the Phytopathological Chemistry of Sweet Potato with Black Rot and Injury.




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A. Sturm
Invertases. Primary Structures, Functions, and Roles in Plant Development and Sucrose Partitioning
Plant Physiology, September 1, 1999; 121(1): 1 - 8.
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