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Articles

Temperature-induced Phase Changes in the Membranes of Glyoxysomes, Mitochondria, and Proplastids from Germinating Castor Bean Endosperm ¹

Alec D. Keith^b

^a Department of Agronomy and Range Science and Department of Vegetable Crops, University of California, Davis, California, 95616, Department of Biophysics, Pennsylvania State University, University Park, Pennsylvania, 16802

Sucrose-gradient purified mitochondria, glyoxysomes, and proplastids from germinating castor bean (Ricinus communis L.) endosperm were examined by electron-spin resonance spectroscopy. A temperature-induced phase change was demonstrated in all of these organelles, their derivative membranes, and in micelles formed from the membrane phospholipids. The apparent transition temperature of the membrane lipids varied slightly between the samples, but in all cases, fell within the temperature range around 10 C where physiological and biochemical changes in the response to temperature for most chilling-sensitive plants occur.

¹ This work was supported in part by Hatch Act Funds, National Science Foundation Grant GB 7868, Dupont Young Faculty Award from E.I. Dupont de Nemours and Co. to R.W.B., Commonwealth Scientific and Industrial Research Organization (Australia) Postdoctoral Award to N.L.W., and Grant AM 1229 from the United States Public Health Service to A.D.K.