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Plant Physiology 54:484-486 (1974)
© 1974 American Society of Plant Biologists

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Articles

Fat Metabolism in Higher Plants

LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts 1

Bruce S. Jacobson2, J. G. Jaworski and P. K. Stumpf

a Department of Biochemistry and Biophysics, University of California, Davis, California 95616

Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.

2 Present address: Department of Biological Sciences, Harvard University, Cambridge, Massachusetts 02138.

1 This research was supported in part by National Institutes of Health Grant GM 19213-02.




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Copyright © 1974 by the American Society of Plant Biologists