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Metabolism of Glutamate in Suspension Cultures of Paul's Scarlet Rose Cells

^a Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069

When specifically labeled glutamate-1-¹⁴C was provided to 4-day-old rose cells, 87.6% of the ¹⁴C in glutamate recovered from protein was in the number 1 carbon atom of the glutamate molecule. It was concluded that newly absorbed glutamate was incorporated directly into protein without any prior metabolism.

A double labeling study with acetate-U-¹⁴C and glutamate-U-³H showed the availability of absorbed glutamate for protein synthesis was equal to that of endogenously synthesized glutamate.