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Plant Physiology 54:532-534 (1974)
© 1974 American Society of Plant Biologists

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Articles

Photosynthetic Activity of Spinach Chloroplasts after Isopycnic Centrifugation in Gradients of Silica 1

J.-J. Morgenthalera,2 and C. A. Pricea

J. Michael Robinsonb,3 and Martin Gibbsb

a Particle Separation Facility, Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey 08903, Department of Biology, Brandeis University, Waltham, Massachusetts 02154

Chloroplast suspensions from spinach (Spinacia oleracea L.) were clearly resolved into intact and stripped chloroplasts by isopycnic centrifugation in density gradients of silica sol ("Ludox") and polyethlene glycol. The intact chloroplasts fixed CO2 and evolved O2 more rapidly than the crude suspensions; the stripped chloroplasts were inactive. During the photosynthetic fixation of 14CO2 in the intact chloroplasts recovered from the gradient, the 14C label was observed to spread through the photosynthetic intermediate pools, as well as into starch, which indicates that the purified chloroplasts are metabolically competent. This appears to be the first report of the retention of photosynthetic activity following the purification of chloroplasts in density gradients.

2 Postdoctoral Fellow of the Foundation for Biomedical Fellowships, Switzerland.

3 Postdoctoral Fellow of the National Institutes of Health.

1 This work was supported in part by the United States Public Health Service Grant HD-05602 to C. A. P. and the United States Atomic Energy Commission Grant AT (11-1)3231 to M. G. Paper of the Journal Series, N. J. Agricultural Experiment Station, Cook College, Rutgers University, New Brunswick, N. J. 08903.






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Copyright © 1974 by the American Society of Plant Biologists