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Plant Physiology 54:586-588 (1974) © 1974 American Society of Plant Biologists Detection and Identification of Cytokinins Produced by Mycorrhizal Fungi 1,2a Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401
Several fungi including six species of the genus Rhizopogon, 22 species of Hebeloma and one of Agaricus have been screened for production of cytokinins. The screening was done by culturing cytokinin-requiring soybean callus tissue alongside the fungus on a medium lacking a cytokinin supply. Growth of the soybean callus indicated production of cytokinins by the fungus. Of the fungi tested, only R. ochraceorubens A. H. Smith gave off sufficient cytokinin to be detected. Although a number of mycorrhizal species are now known to make and give off cytokinins, an even larger number apparently do not do so under the conditions of screening employed. An unidentified ectendotrophic species definitely gave off trans-zeatin, which has been crystallized, and probably trans-ribosylzeatin. Suillus punctipes (Pk.) Sing. apparently produced the same two cytokinins.
3 Present address: Department of Biology, Providence College, Providence, R.I. 02918. 1 This study was supported in part by Grants GB 12115 and GB 35232X from the National Science Foundation. 2 This study represents part of a thesis presented by Carol B. Crafts to the Graduate School of Indiana University in partial fulfillment of the requirements for the Ph.D. degree.
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