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Kinetin-induced Changes in -Aminolevulinic Acid Dehydratase of Tobacco Callus

^a T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington, Kentucky 40506

Kinetin-induced changes in dry weight, soluble protein content, -aminolevulinic acid dehydratase activity, and chlorophyll content of two clones of Nicotiana tabacum L. callus were studied. Kinetin brought about a marked increase in the -aminolevulinic acid dehydratase activity of nongreen tissue just before induction of greening. The experimental data suggested a possible induction of specific chloroplast protein(s) during the kinetin-induced greening of nongreen tobacco tissue. Kinetin caused a decline in the -aminolevulinic acid dehydratase activity and chlorophyll content of the green callus used in the present study.