Plant Physiol.
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Plant Physiology 54:737-743 (1974)
© 1974 American Society of Plant Biologists

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Articles

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

A Double Labeling Determination 1

Graham C. Walkera and Nelson J. Leonarda

Donald J. Armstrongb, Norimoto Muraib and Folke Skoogb

a School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801, Institute of Plant Development, University of Wisconsin, Madison, Wisconsin 53706

Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N6-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with 3H and in the 8-position of the purine ring with 14C. The 3H/14C ratio of N6-benzyladenosine recovered from labeled tobacco callus transfer RNA preparations was compared with the corresponding ratio for N6-benzyladenine supplied in the medium. By this means, with suitable controls, the incorporation of the synthetic cytokinin, N6-benzyladenine, into the transfer RNA of tobacco callus tissue was shown to involve the intact moiety. The observed level of incorporation was up to one N6-benzyladenosine molecule per 104 transfer RNA molecules.

1 This research was supported at the University of Wisconsin by Research Grants GB-25812, GB-35260X, and GB-35260X1 from the National Science Foundation and by the Research Committee of the Graduate School with funds from the Wisconsin Alumni Research Foundation. It was supported at the University of Illinois by Research Grant GM-05829 from the National Institutes of Health.






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Copyright © 1974 by the American Society of Plant Biologists