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An Amphoteric Conjugate of [³H]Gibberellin A₁ from Barley Aleurone Layers ^1,2

^a Department of Vegetable Crops, University of California, Davis, California 95616

The major metabolite produced during incubation of [³H]gibberellin A₁ ([³H]GA₁) with barley aleurone layers is an amphoteric, water-soluble compound tentatively called [³H]ampho GA₁. Formation of [³H]ampho GA₁ in barley aleurones begins after a period of 2.5 hours. As judged by degradation studies as well as Sephadex column chromatography, GA₁ appears to be linked to a peptide; positions C-3 and C-7 were ruled out as conjugation sites.

⁴ Present address: Plant Growth Laboratory, Wickson Hall, University of California, Davis, Calif. 95616.

¹ This research was supported by United States Public Health Service Grant 12885, National Science Foundation Grant 21241, and North Atlantic Treaty Organization Grant 541.

² Ernest Sondheimer's research was characterized by his consistent efforts to provide a chemical basis for an understanding of plant hormone action. He understood that simply measuring a change in concentration of a hormone is not sufficient to explain its function in a process. Not only was he concerned with the occurrence of hormones but also with their identity. The dedication of this volume to mark his lamentably early death should serve to focus the attention of those engaged in growth regulator research on the need to attain a fuller comprehension of the role of hormone biosynthesis and turnover in relation to physiological processes. If this occurs his contribution will remain as much more than a footnote in the annals of hormone research.