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Articles

Enzymatic Esterification of Indole-3-acetic Acid to myo-Inositol and Glucose ¹

^a Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

Incubation of mature sweet corn kernels of Zea mays in dilute solutions of ¹⁴C-labeled indole-3-acetic acid leads to the formation of ¹⁴C-labeled esters of myo-inositol, glucose, and glucans. Utilizing this knowledge it was found that an enzyme preparation from immature sweet corn kernels of Zea mays catalyzed the CoA- and ATP-dependent esterification of indole-3-acetic acid to myo-inositol and glucose. The esters formed were 2-O-(indole-3-acetyl)-myo-inositol, 1-DL-1-O-(indole-3-acetyl)-myo-inositol, di-O-(indole-3-acetyl)-myo-inositol, tri-O-(indole-3-acetyl)-myo-inositol, 2-O-(indole-3-acetyl)-D-glucopyranose, 4-O-(indole-3-acetyl)-D-glucopyranose and 6-O-(indole-3-acetyl)-D-glycopyranose. An assay system was developed for measuring esterification of ¹⁴C-labeled indole-3-acetic acid by ammonolysis of the esters followed by isolation and counting the radioactive indole-3-acetamide.

¹ This work was supported, in part, by the National Science Foundation (GB 18353-X and GB-40821-X). Scintillation counting equipment was provided by the United States Atomic Energy Commission, Contract AT-(11-1)-1338. Journal Article No. 6793 from the Michigan Agricultural Experiment Station.

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