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Some Effects of Applied Gibberellic Acid on the Synthesis and Degradation of Lipids in Isolated Barley Aleurone Layers ¹

^a Michigan State University, Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

An analysis of the lipids in isolated barley (Hordeum vulgare L.) aleurone layers after 12 hours incubation in the presence or absence of gibberellic acid showed no quantitative or qualitative changes. Longer incubation periods resulted in some lipid degradation which was greater in the presence of 1 µM gibberellic acid.

Glycerolipid synthesis was measured in isolated barley aleurone layers during the first 12 hours of incubation in the presence or absence of gibberellic acid by following the incorporation of ³H-glycerol. No significant effect of the hormone was found on either the incorporation of glycerol into lipids or on the types of lipid being synthesized.

¹ This research was supported by the United States Atomic Energy Commission under Contract AT(11-1)-1338.

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