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Plant Physiology 55:120-129 (1975)
© 1975 American Society of Plant Biologists

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Articles

Characterization of Inositol-containing Phosphosphingolipids from Tobacco Leaves

Isolation and Identification of Two Novel, Major Lipids: N-Acetylglucosamidoglucuronidoinositol Phosphorylceramide and Glucosamidoglucuronidoinositol Phosphorylceramide 1

Karan Kaul and Robert L. Lester

a Department of Biochemistry, College of Medicine, University of Kentucky, Lexington, Kentucky 40506

A method for a large scale extraction of phosphoglycosphingolipids from the leaves of Nicotiana tabacum L. has been developed. The phosphosphingolipid concentrate consists of a dozen or more polar lipids as judged by thin layer chromatography. Two of these lipids were purified by chromatography on porous silica beads and partially characterized. These lipids are formulated as: N-acetylglucosamidoglucuronidoinositol phosphorylceramide and glucosamidoglucuronidoinositol phosphorylceramide. Although not fully characterized, the other lipids in the concentrate are inositol-containing phosphosphingolipids with a higher carbohydrate content.

1 This research project was supported by the Tobacco and Health Research Institute, University of Kentucky, Project No. KTRB053.




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Copyright © 1975 by the American Society of Plant Biologists