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Plant Physiology 55:59-63 (1975) © 1975 American Society of Plant Biologists Kinetics and Energetics of Light-driven Chloroplast Glutamine Synthesis 1a Department of Botany, University of California, Davis, California 95616
Chloroplasts contain the enzyme glutamine synthetase. Formation of glutamine by isolated chloroplasts is light-dependent and requires an intact outer envelope. Addition of exogenous glutamic acid, as well as nitrogen donors such as nitrite or ammonium, stimulate the synthesis of this amide. Photosynthetic generation of ATP satisfies the light requirement of glutamine synthesis. The process is supported by cyclic as well as noncyclic photophosphorylation.
2 Present address: Department of Horticulture, Purdue University, West Lafayette, Ind. 47907. 1 This research was supported in part by Grant GB-35464 from the National Science Foundation.
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