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Plant Physiology 55:183-186 (1975)
© 1975 American Society of Plant Biologists

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Articles

Partial Reactions of Photosynthesis in Briefly Sonicated Chlamydomonas

I. Cell Breakage and Electron Transport Activities 1

Virginia A. Curtis, Jerry J. Brand and Robert K. Togasaki

a Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401

The cell structure of Chlamydomonas reinhardi is disrupted by brief exposure to sonication. The extent of cell breakage can be determined quickly by cell count with the light microscope. Rates of photochemical activities of briefly sonicated cells approach those reported for higher plant chloroplasts. These activities are a sensitive function of time of sonication and sonic power used. The method of brief sonication is rapid and convenient and gives a stable preparation useful for determining photochemical activities in Chlamydomonas.

1 This work was supported in part by the National Institutes of Health, United States Public Health Service Grant GM 16314 to A.S.P., and the National Institutes of Health, United States Public Health Service Grant PHS SO5 RR 7031 to R.K.T.




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D. D. Wykoff, J. P. Davies, A. Melis, and A. R. Grossman
The Regulation of Photosynthetic Electron Transport during Nutrient Deprivation in Chlamydomonas reinhardtii
Plant Physiology, May 1, 1998; 117(1): 129 - 139.
[Abstract] [Full Text]


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Copyright © 1975 by the American Society of Plant Biologists