This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Schmid, B. D. Articles by Vanderhoef, L. N. Search for Related Content PubMed PubMed Citation Articles by Schmid, B. D. Articles by Vanderhoef, L. N. Agricola Articles by Schmid, B. D. Articles by Vanderhoef, L. N.

Articles

The Isolation and Characterization of Adenosine Monophosphate-rich Polynucleotides Synthesized by Soybean Hypocotyl Cells

Their Relation to Messenger Ribonucleic Acid ¹

^a Department of Botany, University of Illinois, Urbana, Illinois 61801

Plant ribonucleic acids which have high adenosine monophosphate concentrations were studied. Purified deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid fractions both contained poly-adenosine monophosphate sequences (those from the latter being longer than those from the former); without these poly-adenosine monophosphate sequences their base compositions were the same. The average poly-adenosine monophosphate sequence from purified tenaciously bound ribonucleic acid was 160 residues long, as measured by gel electrophoresis. However, base hydrolysis and chromatography indicated one 3'-nucleoside (adenosine) per 71 nucleotides, giving a chain length of 72 residues. The dominant species in the cytoplasm, as measured by radioactive precursor incorporation, was tenaciously bound ribonucleic acid, whereas deoxyribonucleic acid-like ribonucleic acid was present in greater amounts in the nucleus. This work provides evidence that deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid represent forms of messenger ribonucleic acid in soybean, with deoxyribonucleic acid-like ribonucleic acid residing in the nucleus, perhaps as the messenger ribonucleic acid precursor, and tenaciously bound ribonucleic acid residing, as the active messenger ribonucleic acid, in the cytoplasm.

³ Present address: Agricultural Research, Monsanto Co., St. Louis, Mo. 63166.

⁴ To whom correspondence should be addressed.

¹ This research was supported by National Science Foundation Grant GB-36586, a United States Department of Health, Education and Welfare Biomedical Sciences Support Grant to the University of Illinois, and by a grant from the University of Illinois Graduate Research Board.